By Pearson correlation, the contents of the five compounds in the samples were positively and significantly correlated to their antioxidant activities. The contents of the five marker compounds in Group 1 were higher than Group 2 and also exhibited stronger ABTS activity ( P = 0.005). By UPLC analysis, the raw herbs and two of the granule products (G7 and G12) were allocated into Group 1 and the rest of the granule products into Group 2. HCA and PCA of the TLC analysis clustered the granule products into one group. A 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assay was used to measure the antioxidant capacities of the extracts. Multivariate statistical analysis using hierarchical component analysis (HCA) and principal component analysis (PCA) was used to determine the similarities between the granule products and raw herbs. Five compounds (ginsenosides Rg1, Rg2, Rd and Rb1 and notoginsenoside NR1) in the methanolic extracts were quantified by TLC and UPLC-PDA. MethodsĪqueous extracts of the raw herb (collected from six different sources in China) and granule products (purchased in China, Taiwan and Australia) were re-extracted with methanol to remove water-soluble excipients. This study aims to compare commercially available notoginseng ( Sanqi in Chinese) in both raw and granule forms by thin layer chromatography (TLC) and ultra-performance liquid chromatography with photodiode array detection (UPLC-PDA) using multivariate analysis. However, there have been few studies comparing the quality or efficacy of granules with those of herbal formulations. Granule products produced from medicinal herbs are gaining popularity.
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